USING SPAD 502 PLUS TESTER TO DIAGNOSE CHLOROPHYLL PIGMENT CONTENT IN SPRING WHEAT LEAVES
УДК: 633.111:631.527:581.132 DOI: 10.31588/2073-0462-2026-21-2-22-28 EDN: CSLONA
Keywords:
spring wheat (Triticum aestivum), breeding, varieties, hybrid combinations, pigments, photosynthesis, correlationAbstract
The study was conducted to explore the potential of using the Konica Minolta SPAD 502 Plus contact tester (Japan) to assess photosynthetic pigment content in wheat leaves and to isolate material for breeding. The study was conducted in 2022–2023 in Kirov region. To verify the tester's accuracy, pigment complex composition was analyzed simultaneously using a UVmini-1240 spectrophotometer (Japan). The study subjects included 8 parental varieties and 36 hybrid combinations of the third and fourth generations at the flowering and milky-wax ripeness stages. The values of SPAD 502 Plus tester are characterized by high intervarietal variability (15.4...51.2 device units) with significant genotypic differences (НСР05=4.6...9.3). The manifestation of the trait is distinguished by high varietal specificity in terms of the level of indicator decrease from the flowering phase to milky-wax ripeness (from -3.9 to 56.3%) and is influenced by seasonal effects. A highly significant agreement (r=0.70...0.81) was established between the readings of two compared devices, as well as the possibility of predicting the content of the sum of chlorophyll pigments (Chl a+b) in leaves based on tester measurements (R2=0.82...0.85). The results of paired correlation analysis revealed a significant correspondence between the ranks of genotypes, estimated by the average values by two different methods (rg=0.71...0.77). As a result of research, 10 best hybrid combinations and 3 parental varieties were selected. The hybrid combination Darya x Egisar 29, characterized by a high SPAD 502 Plus value and minimal decline during development, is considered the most promising for breeding. Epos, Darya and Egisar 29 varieties are recommended for inclusion in breeding programs as parental forms due to their high photosynthetic activity. Using SPAD 502 Plus tester allows for rapid assessment of breeding material for chlorophyll pigment content in the field with acceptable accuracy.
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