BIOTECHNOLOGICAL POTENTIAL OF BACTERIAL STRAINS OF PANTOEA BRENNERI TO INCREASE THE CONTENT OF BIOAVAILABLE FORMS OF PHOSPHORUS IN SOIL
DOI 10.12737/2073-0462-2025-10-16 УДК 579.64:631.46
Keywords:
phosphate mobilization, mineralization, solubilization, biopreparations, Pantoea brenneri.Abstract
The studies were conducted to determine the role of strains of soil bacteria Pantoea brenneri in increasing the bioavailability of soil phosphorus and to assess the biotechnological potential of the strains for use as biofertilizers. Soil microcosms were created in four variants: inoculated non-sterile soil (30 g + 5 ml of P. brenneri suspension, 1.5×108 CFU/ml); control with non-sterile soil (30 g + 5 ml of 0.9% NaCl); inoculated sterile soil (30 g + 5 ml P. brenneri suspension, 1.5×108 CFU/ml); control with sterile soil (30 g + 5 ml 0.9% NaCl). Microcosms were incubated for 15 days, samples were taken at the beginning and end of incubation. Phosphate mobilization of P. brenneri strains in soil is confirmed by an increase in the expression level of gdh (glucose dehydrogenase) and pho (phosphatase) genes, the products of which are involved in the solubilization and mineralization of hard-toreach phosphorus compounds. Expression of the target genes in sterile soil was higher than in non-sterile soil, depending on the incubation time and strain used: for the gdh gene - on average by 1.2...1.9 times, for the pho gene - on average by 1.3...1.7 times. The introduction of the strain suspension into the soil contributed to an increase in the number of bioavailable forms of phosphorus in sterile soil by an average of 22...27%, in non-sterile soil - by 15...18%. P. brenneri strains remained viable in the soil throughout the experiment, which characterizes their ability to compete with native soil microflora. On the 5th day of incubation, a 6-8-fold increase in the number of CFU/g of soil was noted, compared with the initial point (day 0), and on the 15th day, the value of this indicator exceeded the initial value by an average of 3...4 times.
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1. Rawat P, Das S, Shankhdhar D. Phosphate-solubilizing microorganisms: mechanism and their role in phosphate solubilization and uptake. Journal of Soil Science and Plant Nutrition. 2020; Vol.21. 49-68 p. doi: 10.1007/s42729-020-00342-7.
2. Timofeeva A, Galyamova M, Sedykh S. Prospects for using phosphate-solubilizing microorganisms as natural fertilizers in agriculture. [Internet]. Plants. 2022; Vol.11. No.16. Article 2119. [cited 2025, May 04]. Available from: https://www.mdpi.com/2223-7747/11/16/2119. doi: 10.3390/plants11162119.
3. Tian J, Ge F, Zhang D. Roles of phosphate solubilizing microorganisms from managing soil phosphorus deficiency to mediating biogeochemical P cycle. [Internet]. Biology. 2021; Vol.10. No. 2. Article 158. [cited 2025, May 04]. Available from: https://www.mdpi.com/2079-7737/10/2/158. doi: 10.3390/biology10020158.
4. Sharma SB, Sayyed R, Trivedi MH. Phosphate solubilizing microbes: Sustainable approach for managing phosphorus deficiency in agricultural soils. [Internet]. SpringerPlus. 2013; Vol.2. No.1. Article 587. [cited 2025, May 04]. Available from: https://link.springer.com/article/10.1186/2193-1801-2-587. doi: 10.1186/2193-1801-2-587.
5. Bhatia S, Sharma D. Biodesulfurization of dibenzothiophene, its alkylated derivatives and crude oil by a newly isolated strain Pantoea agglomerans D23W3. Biochem. Eng. J. 2010; Vol.50. No.3. 104-109 p. doi: 10.1016/j.bej.2010.04.001.
6. Walterson AM, Stavrinides J. Pantoea: insights into a highly versatile and diverse genus within the Enterobacteriaceae. FEMS Microbiol. Rev. 2015; Vol.39. No.6. 968-984 p. doi: 10.1093/femsre/fuv027.
7. Chen Q, Liu S. Identification and characterization of the phosphate-solubilizing bacterium Pantoea sp. S32 in reclamation soil in Shanxi, China. [Internet]. Frontiers in Microbiology. 2019; Vol.10. Article 2171. [cited 2025, May 04]. Available from: https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.02171/full. doi: 10.3389/fmicb.2019.02171.
8. Itkina DL, Suleymanova AD, Sharipova MR. [Pantoea brenneri AS3 and Bacillus ginsengihumi M2.11 as potential biocontrol agents and plant growth promoters]. Mikrobiologiya. 2021; Vol.90. 2. 204-214 p. doi: 10.31857/S0026365621020063.
9. Bulmakova DS, Shagieva GI, Itkina DL. [Antagonistic strains of Pantoea brenneri as plant protection products]. Mikologiya i fitopatologiya. 2023; Vol.57. 5. 352-361 p. doi: 10.31857/S0026364823050033.
10. Suleymanova A, Bulmakova D, Sokolnikova L. Phosphate solubilization and plant growth promotion by Pantoea brenneri soil isolates. [Internet]. Microorganisms. 2023; Vol.11. No.5. Article 1136. [cited 2025, May 04]. Available from: https://www.mdpi.com/2076-2607/11/5/1136. doi: 10.3390/microorganisms11051136.
11. Saadouli I, Mosbah A, Ferjani R. The impact of the inoculation of phosphate-solubilizing bacteria Pantoea agglomerans on phosphorus availability and bacterial community dynamics of a semi-arid soil. [Internet]. Microorganisms. 2021; Vol.9. No.8. Article 1661. [cited 2025, May 04]. Available from: https://www.mdpi.com/2076-2607/9/8/1661. doi: 10.3390/microorganisms9081661.
12. Suleymanova AD, Beinhauer A, Valeeva LR. Novel glucose-1-phosphatase with high phytase activity and unusual metal ion activation from soil bacterium Pantoea sp. strain 3.5.1/t al. Appl. Environ. Microbiol. 2015; Vol.81. No.19. 6790-6799 p. doi: 10.1128/AEM.01384-15.
13. Suleymanova AD, Itkina DL, Pudova DS. [Identification of phytate-hydrolyzing rhizobacteria of Pantoea genus based on phenotypic traits and multilocus analysis]. Mikrobiologiya. 2021; Vol.90. 1. 87-95 p. doi: 10.1134/S0026261721010112.
14. Olsen SR. Estimation of available phosphorus in soils by extraction with sodium bicarbonate. Washington, D.C.: U.S. Dept. of Agriculture. 1954; 19 p.
15. Greiner R, Konietzny U, Jany KD. Purification and characterization of two phytases from Escherichia coli. Arch. Biochem. Biophys. 1993; Vol.303. No.1. 107-113 p. doi: 10.1006/abbi.1993.1261.
16. Strigul NS, Kravchenko LV. Mathematical modeling of PGPR inoculation into the rhizosphere. Environ. Model. Softw. 2006; Vol.21. No.8. 1158-1171 p. doi: 10.1016/j.envsoft.2005.06.003.
17. Pastor-Bueis R, Sanchez-Canizares C, James EK. Formulation of a highly effective inoculant for common bean based on an autochthonous elite strain of Rhizobium leguminosarum bv. phaseoli, and genomic-based insights into its agronomic performance. [Internet]. Frontiers in Microbiology. 2019; Vol.10. Article 2724. [cited 2025, May 04]. Available from: https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.02724/full. doi: 10.3389/fmicb.2019.02724.
18. Tina W, Walde-Meskel E, Wallet F. Symbiotic efficiency of native and exotic rhizobium strains nodulating lentil (Lens culinaris Medik.) in soils of southern Ethiopia. [Internet]. Agronomy. 2016; Vol.6. No.1. Article 11. [cited 2025, May 04]. Available from: https://www.mdpi.com/2073-4395/6/1/11. doi: 10.3390/agronomy6010011.
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